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Mouse IgG1, FITC/RPE/Cy5RPE Conjugated Control


Mouse IgG1, FITC/RPE/Cy5RPE Conjugated Control from Thermo Scientific™ Pierce™ is validated for Ctrl and FACS.

Manufacturer: pierce biotechnology inc  SA112475

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Catalog No. PISA112475

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Description & Specifications

Specifications

Antigen Mouse IgG1 Tri-Color
Applications Control
Applications Flow Cytometry
Conjugate FITC, RPE, RPE-Cy5
Format Purified
Formulation 100 Tests purified isotype control antibody in PBS with 0.2% carrier protein and 0.08% sodium azide
Gene Alias Negative Control, Immunoglobulin Control, Control
Host Species Mouse
Immunogen White blood cells.
Isotype IgG1
Purification Method Affinity chromatography
Quantity 100 TS
Species Reactivity Not Applicable
Storage Requirements Store at 4° C

Mouse IgG1, FITC/RPE/Cy5RPE Conjugated Control from Thermo Scientific™ Pierce™ is validated for Ctrl and FACS.

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody’s species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Host Species: Mouse
Isotype: IgG1
Immunogen: White blood cells.
Formulation: 100 Tests purified isotype control antibody in PBS with 0.2% carrier protein and 0.08% sodium azide
Synonyms: Negative Control, Immunoglobulin Control, Control
This antibody contains a mix of IgG1 (FITC), IgG1 (RPE) and IgG1 (RPE-Cy5) isotypes. SA1-12475 has been successfully used in FACS procedures to assess non-specific binding of mouse monoclonal antibodies in human samples.