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Mouse IgG1 Isotype Control, FITC/RPE/Cy5RPE, Invitrogen™

Mouse IgG1 Isotype Control, FITC/RPE/Cy5RPE conjugate, SA1-12476, from Invitrogen™.

Manufacturer:  Pierce Biotechnology Inc SA112476

Catalog No. PISA112476


 

Specifications

Description

Mouse IgG1 Isotype Control, FITC/RPE/Cy5RPE conjugate, SA1-12476, from Invitrogen.

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody’s species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Host Species: Mouse
Isotype: IgG1, κ
Clone: ZX3
Immunogen: White blood cells.
Formulation: 50 Tests purified isotype control antibody in PBS with 0.2% carrier protein and 0.08% sodium azide
Synonyms: Negative Control, Immunoglobulin Control, Control
This antibody contains a mix of IgG1 (FITC), IgG1 (RPE) and IgG1 (RPE-Cy5) isotypes. SA1-12476 has been successfully used in FACS procedures to assess non-specific binding of mouse monoclonal antibodies in human samples.
References:
Test: Flow, Species: Hu, Reference: The evaluation of leukocytes in response to the in vitro testing of ventricular assist devices. (Sep 2013) Artif Organs, Vol: 37, 9, 793-801, Chan CH,Hilton A,Foster G,Hawkins KM,Badiei N,Thornton CA; Pubmed ID: 23981196
Description & Specifications

Specifications

Mouse IgG1
ZX3
50 Tests purified isotype control antibody in PBS with 0.2% carrier protein and 0.08% sodium azide
Negative Control, Immunoglobulin Control, Control
White blood cells.
Affinity chromatography
DO NOT FREEZE!
Control,Flow Cytometry
FITC, RPE, RPE-Cy5
Purified
Murine
IgG1, κ
50 Tests