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anti-IL-6, Polyclonal, Novus Biologicals™

$117.00 - $246.00

Specifications

Host Species Rabbit
Immunogen Recombinant human IL-6 produced in E. coli.
Antigen IL-6
Monoclonal or Polyclonal Polyclonal
Format Antiserum
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 Disclaimers

For Research Use Only

Products
Catalog Number Mfr. No. Conjugate Quantity Price Quantity    

NB6001131

 
novus biologicals canada
NB600-1131
Unlabeled 0.2mL Each for $246.00

NB6001131SS

 
novus biologicals canada
NB600-1131SS
Unlabeled 0.04mL Each for $117.00
Description & Specifications

Specifications

Host Species Rabbit
Immunogen Recombinant human IL-6 produced in E. coli.
Antigen IL-6
Monoclonal or Polyclonal Polyclonal
Format Antiserum
Primary or Secondary Primary
Regulatory Status RUO
Applications ELISA
Applications Immunocytochemistry
Applications Immunofluorescence
Applications Immunohistochemistry
Applications Immunohistochemistry (Paraffin)
Applications Immunoprecipitation
Applications Western Blotting
Test Specificity This antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity. Cross-reactivity reported in scientific literature: Mouse - PMID 23015436; Rat - PMID 25058444 .
Gene Accession No. P05231
Gene ID (Entrez) 3569
Formulation 0.02M Potassium phosphate with 0.15M Sodium Chloride pH 7.2