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anti-IL-6, Polyclonal, Novus Biologicals™


Manufacturer: novus biologicals canada  NB600-1131SS

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 Disclaimers

For Research Use Only

Catalog No. NB6001131SS

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Description & Specifications

Specifications

Antigen IL-6
Applications ELISA
Applications Immunocytochemistry
Applications Immunofluorescence
Applications Immunohistochemistry
Applications Immunohistochemistry (Paraffin)
Applications Immunoprecipitation
Applications Western Blotting
Conjugate Unlabeled
Cross Reactivity Human
Cross Reactivity Mouse
Cross Reactivity Rat
Format Antiserum
Formulation 0.02M Potassium phosphate with 0.15M Sodium Chloride pH 7.2
Gene Accession No. P05231
Host Species Rabbit
Immunogen Recombinant human IL-6 produced in E. coli.
Quantity 0.04mL
Regulatory Status RUO
Primary or Secondary Primary
Monoclonal or Polyclonal Polyclonal
Gene ID (Entrez) 3569
Test Specificity This antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity. Cross-reactivity reported in scientific literature: Mouse - PMID 23015436; Rat - PMID 25058444 .