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anti-IL-6, Polyclonal, Novus Biologicals™

Manufacturer:  Novus Biologicals Canada NB6001131SS

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Catalog No. NB6001131SS


 

Disclaimers:

For Research Use Only


Specifications Description & Specifications

Specifications

IL-6
Unlabeled
P05231
Rabbit
0.04mL
Primary
3569
Human,Murine,Rat
ELISA,Immunocytochemistry,Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Immunoprecipitation,Western Blot
0.02M Potassium phosphate with 0.15M Sodium Chloride pH 7.2
Antisera
Recombinant human IL-6 produced in E. coli.
RUO
Polyclonal
This antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity. Cross-reactivity reported in scientific literature: Mouse - PMID 23015436; Rat - PMID 25058444 .