anti-IL-4 APC, Clone: 11B11, EMD Millipore™
Rat Monoclonal Antibody
Manufacturer: Millipore Sigma MABF1504
Specifically detects IL-4 Clone: 11B11 in Mouse samples, and it is validated for Flow CytometryThe 11B11 antibody binds to mouse Interleukin-4 (IL-4), a 14kDa cytokine that is largely secreted by activated T cells of the Th2 subset, and to some degree by NKT and mast cells. This cytokine acts as a stimulatory factor for B cells, inducing their proliferation and differentiation, as well as playing a role in immunoglobulin class-switching. IL-4 may also provide autocrine stimulation for T cells, and affect the function of antigen presenting cells such as macrophages and dendritic cells. IL-4 can bind and signal via three cell surface receptor types: CD124 by itself, CD124 in combination with the common gamma chain (type I complex), or CD124 combined with CD213a1 (type II complex).The 11B11 antibody is widely used for detection of intracellular levels of IL-4 protein by flow cytometry, as well as for analysis of soluble cytokine as measured by ELISA, and in functional assays to neutralization cytokine-receptor interactions.
|Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.|
|Purified by size-exclusion chromatography|
|2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze|
For Research Use Only