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anti-DAAM2, Polyclonal, Novus Biologicals™

Manufacturer: novus biologicals canada  NBP2-24536SS

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For Research Use Only

Catalog No. NBP224536SS


Description & Specifications


Antigen DAAM2
Applications Western Blotting
Concentration 0.5mg/mL
Conjugate Unlabeled
Cross Reactivity Human
Cross Reactivity Mouse
Format Affinity Purified
Formulation 100μg antibody in 200μL PBS containing 0.05% BSA
Host Species Rabbit
Immunogen A portion of amino acids 100-150 of human DAAM2 was used as the immunogen for this antibody.
Quantity 0.025mg
Regulatory Status RUO
Primary or Secondary Primary
Monoclonal or Polyclonal Polyclonal
Gene ID (Entrez) 23500
Test Specificity The amino acid sequence used as immunogen for this antibody is 100% homologous in human and rhesus monkey, 94% homologous in horse, 88% homologous in dog, and 76% homologous in cow, mouse and rat. For IF, the GLI1 IMG-6553A antibody was used at 10 ug/ml (Hu, 2010). Protocol: Cells were washed in PBS and fixed in 4% PFA, pH 7.4 (20g PFA in 500 ml PBS) for 10 min. The fixed cells were permeabilized in 0.5% Triton X-100 (in PBS) for 5 min and blocking in 50 ul of 20% HINGS (heat inactivated normal goat serum diluted in PBS) for 1 hr. The GLI1 antibody was incubated at 37 degrees C for 1 hr or at 4 degrees C overnight. Following 3 X 5 min washes in PBS, an AlexaFluor488 or 594-conjugated goat anti-rabbit antibody was used as a second step (diluted in 20% HINGS at 1:1000 for 1 hr at room temperature i the dark). Following 3 X 5 min washes in PBS, the slides were mounted with DABCO (1,4-diazabicyclo[2.2.2]octane) anti-fading agent for fluorescence microscopy.