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anti-BID, Polyclonal, Novus Biologicals™

$160.00 - $278.00

Specifications

Host Species Rabbit
Immunogen Full-length recombinant mouse Bid protein was used as immunogen.
Antigen BID
Monoclonal or Polyclonal Polyclonal
Format Antiserum
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 Disclaimers

For Research Use Only

Products
Catalog Number Mfr. No. Conjugate Quantity Price Quantity    

NB10056106

 
novus biologicals canada
NB100-56106
Unlabeled 0.05mL Each for $278.00

NB10056106T

 
novus biologicals canada
NB100-56106SS
Unlabeled 0.025mL Each for $160.00
Description & Specifications

Specifications

Host Species Rabbit
Immunogen Full-length recombinant mouse Bid protein was used as immunogen.
Antigen BID
Monoclonal or Polyclonal Polyclonal
Format Antiserum
Primary or Secondary Primary
Regulatory Status RUO
Applications Immunohistochemistry
Applications Immunohistochemistry (Frozen)
Applications Immunohistochemistry (Paraffin)
Applications Immunoprecipitation
Applications Western Blotting
Test Specificity Full-length Bid is known to undergo cleavage/truncation (reviewed in Yin, 2006). Bid was initially found to be cleaved and activated by capspase-8 following death receptor activation. The term “Bid” was first used to described the caspase-8 cleaved/truncated C-terminal Bid. Bid can be also cleaved by other proteases such as Granzyme B, calpains and cathepsin. The C-terminal portion is considered to be the active Bid moiety. This active form can translocate from the cytosol to the mitochondria. Therefore, the appearance of Bid in the mitochondria is considered to be an indication of active Bid. However, it should also be noted that Bid has been shown to translocate to the mitochondria without cleavage in some model systems. The proteolytic cleavage of Bid usually occurs in the unstructured loop region between the alpha 2 and alpha 3 helices, which is between amino acids (aa) 41 and 79 of Bid. For example, the caspase-8/3 cleavage site is at 60 (human) and 59 (mouse). It should be noted that after cleavage, the smaller N-terminal portion of Bid is not necessarily separated from the larger C-terminal portion. The exact size of the C-terminal cleavage products depends on the Bid cleavage sites; generally 11-15 kDa C-truncated cleavage forms are generated. Shorter C-terminal Bid cleavage products may result from protease cleavage that involves both caspases and other proteases. Full length Bid is quite stable, but cleaved/truncated forms may have a short half-life (less than 1.5 h) and degrade rapidly. Therefore, a time course may be useful when detecting Bid cleavage fragments.
Gene ID (Entrez) 637
Formulation Neat serum