BDNF Polyclonal Antibody, Invitrogen™

Description

By ELISA, this antibody exhibits a cross-reactivity of less than 1% to recombit human BDNF, NT3 and NT4/5. Use at 1-10 µg/mL for inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). Reconstitute with 500 µL of distilled water. After reconstitution keep aliquots at -20° for a higher stability, and at 4°C with an appropriate antibacterial agent.

BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer′s and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.