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eBioscience™ Annexin V, PE, Recombinant Protein

Recombinant Protein

$130.50 - $274.05

Specifications

Name Annexin V
Recombinant Yes
Conjugate PE
Purity >89%
Purification Method SDS-gel electrophoresis and reverse phase HPLC
View More Specs
Products
Catalog Number Mfr. No. Quantity Price Quantity    

509291

 
ebioscience™
BMS306PE100
100 tests Each for $274.05

509292

 
ebioscience™
BMS306PE/20
20 tests Each for $130.50
Description & Specifications

Specifications

Name Annexin V
Recombinant Yes
Conjugate PE
Purity >89%
Purification Method SDS-gel electrophoresis and reverse phase HPLC
Source E. coli
For Use With (Application) Flow Cytometric Analysis
Molecular Weight 35.8kDa
Storage Requirements Annexin V-PE is stable at 2-8deg;C as indicated on the label. Do not freeze!

Annexins are a family of calcium-dependent phospholipid-binding proteins, which bindto phosphatidylserine (PS) to identify apoptotic cells. In healthy cells, PS is predominantlylocated along the cytosolic side of the plasma membrane. Upon initiation of apoptosis,PS loses its asymmetric distribution in the phospholipid bilayer and translocates tothe extracellular membrane, which is detectable with fluorescently labeled AnnexinV. In early stages of apoptosis, the plasma membrane excludes viability dyes such aspropidium iodide (PI) and 7-AAD, therefore cells which display only Annexin V staining(PI/7-AAD negative) are in early stages of apoptosis. During late-stage apoptosis, loss ofcell membrane integrity allows Annexin V binding to cytosolic PS, as well as cell uptakeof PI and 7-AAD. Annexin V staining, paired with 7-AAD or PI is widely used to identifyapoptotic stages by flow cytometry.