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eBioscience™ Annexin V, FITC, Recombinant Protein

Recombinant Protein

$130.50 - $398.75

Specifications

Name Annexin V
Recombinant Yes
Conjugate FITC
Purity >89%
Purification Method SDS-gel electrophoresis and reverse phase HPLC
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Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
509288 Life Technologies
LSBMS306FI100
100 tests Each for $274.05
509289 Life Technologies
LSBMS306FI20
20 tests Each for $130.50
509290 Life Technologies
LSBMS306FI300
300 tests Each for $398.75
Specifications

Description

Annexins are a family of calcium-dependent phospholipid-binding proteins, which bindto phosphatidylserine (PS) to identify apoptotic cells. In healthy cells, PS is predominantlylocated along the cytosolic side of the plasma membrane. Upon initiation of apoptosis,PS loses its asymmetric distribution in the phospholipid bilayer and translocates tothe extracellular membrane, which is detectable with fluorescently labeled AnnexinV. In early stages of apoptosis, the plasma membrane excludes viability dyes such aspropidium iodide (PI) and 7-AAD, therefore cells which display only Annexin V staining(PI/7-AAD negative) are in early stages of apoptosis. During late-stage apoptosis, loss ofcell membrane integrity allows Annexin V binding to cytosolic PS, as well as cell uptakeof PI and 7-AAD. Annexin V staining, paired with 7-AAD or PI is widely used to identifyapoptotic stages by flow cytometry.
Description & Specifications

Specifications

Annexin V
FITC
SDS-gel electrophoresis and reverse phase HPLC
Flow Cytometric Analysis
Annexin V-FITC is stable at 2-8deg;C as indicated on the label. Do not freeze!
Yes
>89%
E. coli
35.8kDa