Invitrogen™ AMPK (A2/B2/G1) Recombinant Human Protein
AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis. AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex.
Manufacturer: Invitrogen™ LSPV6248
AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signaling pathways in response to adiponectin, leptin and CAMKK beta. Recombinant full-length human AMPK (combination of A2/B2/G1 subunits) was expressed by baculovirus in Sf9 insect cells using C-terminal His tags.
- Validated in LanthaScreen™ Kinase Binding Assay
- Protocols available for simple use and optimized performance
- Multiple pack sizes available for convenient use
|Target & Lead Identification & Validation|Kinase Biology|Kinase Proteins & Expression Reagents|Protein Biology|Enzyme & Protein Activity Assays|Kinase Activity|Protein Assays and Analysis|Industrial & Applied Science|Pharma & Biopharma|
|Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.|
|50mM sodium phosphate pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.2mM DTT, 25% glycerol|