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Invitrogen™ AMPK (A1/B2/G1) Recombinant Human Protein DFS Item

AMPK (A1/B2/G1) is a heterotrimeric protein consisting of an α catalytic subunit, and non-catalytic β and γ subunits. Recombinant full-length human AMPK (combination of A1/B2/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tags.

Manufacturer:  Invitrogen™ LSPV6245

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Catalog No. PV6245



Description

Description

AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress. AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and hydroxy beta-methylglutaryl-CoA reductase. The beta subunit may be a positive regulator of AMPK activity and is highly expressed in skeletal muscle.

  • Validated in LanthaScreen™ Kinase Binding Assay
  • Protocols available for simple use and optimized performance
  • Multiple pack sizes available for convenient use
Specifications

Specifications

Target & Lead Identification & Validation|Kinase Biology|Kinase Proteins & Expression Reagents|Protein Biology|Enzyme & Protein Activity Assays|Kinase Activity|Protein Assays and Analysis|Industrial & Applied Science|Pharma & Biopharma
63.6/36/40kDa
Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.
aa 10-end
Yes
50mM sodium phosphate pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.2mM DTT, 25% glycerol
100μg
Kinase Assay
Human
His Tag
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