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AMPK A1/B1/G1 Recombinant Human Protein DFS Item

AMPK A1/B1/G1 Recombinant Human Protein

Manufacturer:  Life Technologies LSPV4672

Catalog No. PV4672



Description

Description

AMP-activated protein kinase (AMPK) is a heterotrimeric complex that acts as sensor of cellular energy levels. The signaling cascades initiated by activating AMPK are critical to regulating metabolic events in the liver, skeletal muscle, heart, adipose tissue, and pancreas. An impairment in fuel metabolism that occurs in obesity is a factor leading to type 2 diabetes. The insulin resistance associated with type 2 diabetes is most profound at the level of skeletal muscle, the primary site of glucose and fatty acid utilization. Activation of AMPK is of interest for the treatment of type 2 diabetes.
Specifications

Specifications

NP_006242.4, NP_006244.2, NP_002724.1
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
AMPK A1/B1/G1 Recombinant Human Protein
10μg
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
AMPK A1/B1/G1 Recombinant Human Protein
Kinase assay
Baculovirus
AMPK A1/B1/G1 Recombinant Human Protein
Human
0.34mg/mL
90.8/57.8/41.7kDa
90% by SDS-PAGE
–80°C
1 Each
AMPK A1/B1/G1
AMPK (A1/B1/G1) was pre-diluted in enzyme dilution buffer and assayed in 25mM HEPES (pH 7.5), 10mM MgCl2, 0.5mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 500μM ATP, 200μM SAMS peptide substrate (HMRSAMSGLHLVKRR), 150μM AMP and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinase and inhibitors
CAMKL
14100nmoles of phosphate transferred to SAMS peptide substrate (HMRSAMSGLHLVKRR) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.083μg/mL. Activity increased 1.5 fold by using 150μM AMP
GST Tag, His Tag
Documents

For Research Use Only. Not for use in diagnostic procedures.