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Invitrogen™ Ambion™ ULTRAhyb™ Ultrasensitive Hybridization Buffer with Manual DFS Item

Proprietary blend of hybridization accelerators and blocking agents to enhance the levels of hybridization

Manufacturer: invitrogen™  AM8669M

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Catalog No. AM8669M


Description & Specifications


Buffer Hybridization
Quantity 4 × 125mL
Storage Requirements 4°C

  • Signals that once took days to visualize are now apparent in hours
  • It is supplied in four bottles containing 125mL each
  • Increase the sensitivity of any blot hybridization experiment
  • Increase random-primed DNA probe sensitivity up to 100x; RNA probe sensitivity 20x
  • Decrease hybridization time to 2 hours
  • Using standard hybridization buffers, only 1 to ;5% of target molecules on a blot hybridizes to probe, making blots a relatively insensitive method for nucleic acid analysis (Vernier et al. (1996) Anal Biochem 235: 11-19): With ULTRAhyb™ Ultrasensitive Hybridization Buffer, the hybridization reaction approaches completion, so that as few as 10,000 molecules can be detected
  • Compatible with RNA and DNA probes labeled isotopically and nonisotopically in northerns, Southerns, and dot/slot blots
  • Contains 50% formamide and is compatible with positively charged membranes
  • Requires 10-100x less nonisotopic DNA probe than standard hybridization buffers, making it possible to perform many more hybridizations with a single labeling reaction


DNA and RNA Purification and Analysis, Northern Blotting, Nucleic Acid Gel Electrophoresis and Blotting

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