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Invitrogen™ Ambion™ TURBO™ DNase (2U/μL) DFS Item


Efficiently degrades and digests DNA

Manufacturer: invitrogen™  AM2238

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Catalog No. AM2238

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Description & Specifications

Specifications

Quantity 1000 units
Storage Requirements TURBO DNase and 10X Reaction Buffer should be stored at -20°C

Cleaves double-stranded DNA nonspecifically to leave 5' phosphorylated oligodeoxynucleotides with an increased affinity for DNA-binding and remains active in presence of salt.

  • Up to 50X more activity and 350% greater catalytic efficiency
  • Efficiently degrades DNA in solutions containing up to 0.25M salt
  • Efficiently digests DNA to oligonucleotides
  • Used in clearing DNA templates from in vitro transcription reactions
  • RNase-free and recombinant in origin

  • Used to clear DNA contamination from RNA samples prior to RT-PCR
  • Conventional DNase I has a poor affinity for DNA and cleaves DNA of low concentration
  • Salt-sensitive; 20 mM NaCl can reduce activity of enzyme by 30%
  • Purified from bovine pancreas, one of richest natural sources of RNase A
  • Threat of contaminating RNase activity in DNase I preparations requires that enzyme be exhaustively purified

TURBO DNA-free (Mfr. No. AM1907) is available. It contains TURBO DNase along with reagents to inactivate the enzyme and remove divalent cations from the sample post-digestion.

PCR & Real-Time PCR, Real Time PCR (qPCR), Reverse Transcription