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Ambion™ T7 RNA Polymerase (cloned) 20U/μL DFS Item


A cloned high-purity RNA polymerase with a high specificity for its promoter

Manufacturer: life technologies  AM2084

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Catalog No. AM2084

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Description & Specifications

Specifications

Concentration 20U/μL
For Use With (Application) Synthesizing high specific activity RNA hybridization probes, biologically active mRNA, and antisense RNA
Includes One tube containing 30,000 units at a concentration of 20U/μL
Quantity 30,000 units
Sample Type DNA
Storage Requirements Store at -20deg.C

  • This enzyme will transcribe large amounts of RNA from DNA sequences downstream of the promoter and show no cross talk between promoters
  • One tube containing 5000U at a concentration of 20U/μL is provided. Its high purity makes it ideal for synthesizing high specific activity RNA hybridization probes, biologically active mRNA, and antisense RNA
  • The RNA Polymerase is rigorously tested for contaminating nonspecific endonuclease, exonuclease, protease, and RNase activity
  • It is also tested for functionality with the MAXIscript Kit at 20U per reaction
  • Unit Definition: One unit of RNA Polymerase will catalyze the incorporation of 1nmol of nucleoside triphosphate into acid-insoluble material in 60 minutes at 37°C

DNA & RNA Purification & Analysis, Gene Expression Analysis & Genotyping, in vitro Transcription, Nucleic Acid Labeling & Oligo Synthesis, RNA Labeling