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Invitrogen™ Ambion™ RNase I (cloned) 100U/μL DFS Item

Efficiently cleaves after all four bases of single-stranded RNA, in contrast to RNase A, which only cleaves after C and U residues

Manufacturer: invitrogen™  AM2295

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Catalog No. AM2295

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Description & Specifications


Concentration 100U/μL
Quantity 25,000 units
Storage Requirements Store at -20°C

RNase I degrades all RNA dinucleotide bonds leaving a 5' hydroxyl and 2', 3' cyclic monophosphate. RNase I will degrade any RNA to mixture of mononucleotides, dinucleotides, and trinucleotides and does not degrade DNA, although it will bind to DNA. It has marked preference for single-stranded RNA over double-stranded RNA, which allows it to work well in ribonuclease protection assays.

  • Supplied in one tube of 10,000U (100U/μL)
  • Endonuclease-free, exonuclease-free, protease-free
  • Rigorously tested for nonspecific contaminating endonuclease, exonuclease, and protease activity
  • Unit Definition: One unit is amount of enzyme required to produce 1μg of acid-soluble material from mouse liver RNA in 30 min. at 37°C

DNA & RNA Purification & Analysis, Nuclease Protection Assays, Nucleic Acid Gel Electrophoresis & Blotting