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Invitrogen™ Ambion™ RNAqueous Total RNA Isolation Kit DFS Item

Uses glass fiber filter separation technology

Manufacturer: invitrogen™  AM1912M

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Catalog No. AM1912M


Description & Specifications


Final Product Type Total RNA
For Use With (Application) Northern Blotting, Nuclease Protection Assays, RT-PCR (Endpoint), Real-Time PCR, cDNA Library Construction
Includes 6mL Formaldehyde Load Dye; 60mL Lysis/Binding Solution; 60mL Water for 64% Ethanol; 4mL Lithium Chloride Precipitation Solution; 40mL Wash Solution 1; 80mL Wash Solution 2/3 Concentrate; 50 Filter Cartridges; 100 Collection Tubes; 10mL Elution Solution; Manual
Isolation Technology Spin Column (Glass Fiber Filter)
Product Type Kit with manual
Quantity 50 preps
Sample Type Bacteria, Cells, Plant Samples, Tissue, Viral Samples, Yeast
Target Total RNA

The RNAqueous Kit is for phenol-free total RNA isolation from 0.1g to 0.5g of tissue or from 107 to 108 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

  • Convenient syringe-based filtration
  • Compatibility with vacuum manifolds when processing multiple samples
How it works
The RNAqueous Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.

Not high-throughput compatible (Manual)


DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues