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Invitrogen™ Ambion™ RNAqueous-Midi Total RNA Isolation Kit DFS Item


RNA isolation for midrange amounts of starting material

Manufacturer: invitrogen™  AM1911M

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Catalog No. AM1911M

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Description & Specifications

Specifications

Final Product Type Total RNA
For Use With (Application) Northern Blotting, Nuclease Protection Assays, RT-PCR (Endpoint), Real-Time PCR, cDNA Library Construction
Includes Formaldehyde Load Dye, Lysis/Binding Solution, Water for 64% Ethanol, Lithium, Chloride Solution, Wash Solution No. 1 and Wash Solution 2/3 Concentrate, Syringe Filter Units, Collection Tubes and Elution Solution, Manual
Isolation Technology Syringe Filter (Glass Fiber)
Product Type Kit with manual
Quantity 15 preps
Sample Type Tissue Cells
Target Total RNA

The Ambion RNAqueous-Midi Kit is for phenol-free total RNA isolation from 0.1g to 0.5g of tissue or 107 to 108 cells using a guanidinium-based lysis/;denaturant and glass fiber filter separation technology. The RNAqueous-Midi Kit provides enough reagents for 15 RNA purifications. The Ambion RNAqueous-Midi Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.

  • Convenient syringe-based filtration
  • Compatible with vacuum manifolds when processing multiple samples

Not high-throughput compatible (Manual)

Applications:

DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues