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Invitrogen™ Ambion™ RNAqueous-Midi Total RNA Isolation Kit DFS Item


RNA isolation for midrange amounts of starting material

Manufacturer: invitrogen™  AM1911

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Catalog No. AM1911

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Description & Specifications

Specifications

Final Product Type Total RNA
For Use With (Application) Northern Blotting, Nuclease Protection Assays, RT-PCR (Endpoint), Real-Time PCR, cDNA Library Construction
Includes Formaldehyde Load Dye, Lysis/Binding Solution, Water for 64% Ethanol, Lithium, Chloride Solution, Wash Solution No. 1 and Wash Solution 2/3 Concentrate, Syringe Filter Units, Collection Tubes and Elution Solution
Isolation Technology Syringe Filter (Glass Fiber)
Product Type Kit
Quantity 15 preps
Sample Type Tissue Cells
Target Total RNA

The Ambion RNAqueous-Midi Kit is for phenol-free total RNA isolation from 0.1g to 0.5g of tissue or 107 to 108 cells using a guanidinium-based lysis/;denaturant and glass fiber filter separation technology. The RNAqueous-Midi Kit provides enough reagents for 15 RNA purifications. The Ambion RNAqueous-Midi Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.

  • Convenient syringe-based filtration
  • Compatible with vacuum manifolds when processing multiple samples

Not high-throughput compatible (Manual)

Applications:

DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues