Ambion RNAsecure™ (patents pending) is a unique non-enzymatic reagent that will irreversibly inactivate RNases in solution.
No post-treatment autoclaving
Safe, easy-to-use reagent inactivates RNases in solutions
Inactivation process can be repeated to protect against newly introduced contaminants
Compatible with downstream procedures, such as RT-PCR and in vitro transcription
Exonuclease Activity: 5' - 3'
Inactivate RNases in Tris and other solutions that cannot be treated with DEPC RNase contamination in reagents used for RNA isolation and analysis can contribute to experimental inconsistency and, at worst, can cause experimental failure. Traditionally, DEPC has been used to treat solutions that come into contact with RNA. DEPC treatment is time-consuming, possibly hazardous, and only eliminates RNases present at the time of DEPC treatment. In addition, some solutions (e.g., primary amine containing compounds such as Tris) cannot be treated with DEPC at all. Finally, DEPC has to be inactivated by autoclaving post-treatment to prevent it from interfering with downstream enzymatic reactions. RNAsecure Reagent eliminates these problems. Unlike DEPC, RNAsecure can be used on virtually any solution and does not require post-treatment autoclaving. A unique feature of RNAsecure is that reheating after the initial treatment will reactivate the RNase-destroying agent to eliminate any new contaminants. RNAsecure is supplied as a 25X concentrated stock. It is added to solutions as part of reactions in vitro transcription, RT-PCR, etc.), prior to the addition of enzymes, and heated to 60°C for 10 minutes to inactivate RNase.
DNA and RNA Purification and Analysis, General RNA Purification Reagents and Accessories, PCR and Real-Time PCR, RNA Extraction, Real Time PCR (qPCR), Total RNA Isolation, Total RNA from Animal Cells and Tissues, mRNA Isolation