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Invitrogen™ PureLink™ RNA Mini Kit 
Provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol
Manufacturer: Invitrogen™ LS12183025
Description
Rapid and convenient column purification
The PureLink™ RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1000μg of purified RNA from a single extraction. High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination.
Simplified, nontoxic RNA purification
The PureLink™ RNA Mini Kit combines nontoxic guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can typically be completed in less than 20 minutes without the need for hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl, or alcohol precipitation.
- Obtain high-yield, high-integrity RNA
- Rapid, non-hazardous RNA purification capabilities
- Ready-to-use RNA for many downstream applications
Specifications
| Total RNA | |
| Total RNA | |
| Spin Column | |
| 250 preps | |
| • Lysis Buffer (500 ml) • Wash Buffer I (250 ml) • Wash Buffer II (75 ml) • RNase-Free Water (75 ml) • Spin Cartridges with collection tubes (5 × 50 each) • Collection Tubes (5 × 50 each) • Recovery Tubes (5 × 50 each) Store at room temperature. |
|
| 30 µl | |
| 20 min | |
| Room Temperature | |
| PureLink™ |
| Microarray Analysis, Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting | |
| Any Tissue Type, Bacterial Culture, Cell Cultures, Plant, Tissue Homogenates, Cultured Cells, Yeast, Tissue Sections, Blood, Liquid Samples (e.g. Serum) | |
| Silica Spin Column | |
| 350 µg | |
| 100 µl | |
| Not High-throughput Compatible (Manual) | |
| Mini | |
| 5x10^6 to 1x10^8 cells, Up to 200 mg of tissue | |
| PureLink™ |
Promotions
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Get Gene Expression Products with Purchase
Spend up to $5,000 on select gene expression products and get a matching amount at no additional cost.: 06/30/21
For Research Use Only. Not for use in diagnostic procedures.