Invitrogen™ PureLink™ RNA Mini Kit
Provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol
Manufacturer: Invitrogen™ LS12183025
Rapid and convenient column purification
The PureLink™ RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1000μg of purified RNA from a single extraction. High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination.
Simplified, nontoxic RNA purification
The PureLink™ RNA Mini Kit combines nontoxic guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can typically be completed in less than 20 minutes without the need for hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl, or alcohol precipitation.
- Obtain high-yield, high-integrity RNA
- Rapid, non-hazardous RNA purification capabilities
- Ready-to-use RNA for many downstream applications
|• Lysis Buffer (500 ml)
• Wash Buffer I (250 ml)
• Wash Buffer II (75 ml)
• RNase-Free Water (75 ml)
• Spin Cartridges with collection tubes (5 × 50 each)
• Collection Tubes (5 × 50 each)
• Recovery Tubes (5 × 50 each)
Store at room temperature.
|Microarray Analysis, Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting|
|Any Tissue Type, Bacterial Culture, Cell Cultures, Plant, Tissue Homogenates, Cultured Cells, Yeast, Tissue Sections, Blood, Liquid Samples (e.g. Serum)|
|Silica Spin Column|
|Not High-throughput Compatible (Manual)|
|5x10^6 to 1x10^8 cells, Up to 200 mg of tissue|
For Research Use Only. Not for use in diagnostic procedures.