missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Please login to your online account to display your discounted pricing
Invitrogen™ mRNA Catcher™ PLUS Purification Kit 
The mRNA Catcher™ PLUS Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format.
Supplier: Invitrogen™ K157002
Description
The mRNA Catcher™ PLUS Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format. By taking advantage of Locked Nucleic Acid (LNA) technology, the mRNA Catcher PLUS Kit provides improved performance and a faster protocol, while preserving mRNA yield and purity.
How it works
mRNA from total RNA, cells, tissue, and blood samples is hybridized to an immobilized, single-stranded 20-mer oligo(dT)-containing LNA. The incorporation of LNA into the oligo probe increases the hybridization efficiency and specificity of the mRNA binding. The mRNA can either be eluted or directly reversed-transcribed to cDNA directly in the well. The mRNA Catcher PLUS method does not require centrifugation, precipitation, phase separation, or column filtration. In addition, the entire method is amenable to automation for streamlined, high-throughput expression profiling for large-scale processes. Isolated mRNA is suitable for use in downstream applications such as RT-PCR and qRT-PCR.
• Automated protocols, high-throughput compatible
• Robust performance: high binding capacity per well (>80ng/well)
• Reproducible results: low CVs from well-to-well and plate-to-plate (â½ 2%)
• Optimized protocols: for small sample sizes (100 cells, 4 mg tissue, and 40 μL blood)
• Compatible plate design: may be used with most PCR cyclers
Specifications
| • 1 x 96-well mRNA Catcher™ PLUS Plate; store at 4°C • 4 mL 2X Lysis Buffer L20; room temperature • 30 mL Wash Buffer W15; room temperature • 4 mL Dilution Buffer DL1; room temperature • 8 mL Elution Buffer E3 (DEPC-treated RNase-free water); room temperature • 100 μL 0.5M DTT; 4°C |
|
| 1.5 hr. (Including sample homogenization) | |
| Cells, Tissue, Total RNA, Whole Blood | |
| High-throughput Compatible | |
| RT-PCR, qPCR, cDNA Library Construction, Microarray Analysis | |
| Room Temperature | |
| >80 ng |
| Oligo dT Affinity | |
| 80 μL | |
| mRNA | |
| Robotic Liquid Handling Systems | |
| 96 Preps | |
| Cells: 100–106 Tissue: 4–40 mg Total RNA: 0.1–100 μg Whole Blood: 40 μL |
For Research Use Only. Not for use in diagnostic procedures.