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Ambion™ Mouse RiboPure™-Blood RNA Isolation Kit DFS Item

High-quality RNA isolation from mouse and rat blood

Manufacturer: life technologies  AM1951

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Catalog No. AM1951

  • / Each

Description & Specifications


Final Product Type Total RNA, micro RNA
For Use With (Application) Microarray Analysis, Northern Blotting, RT-PCR (Endpoint), Real-Time PCR
Includes Acid–Phenol:Chloroform, 3M sodium acetate pH 5.5, wash solution 1 concentrate, wash solution 2/3 concentrate, 2mL tubes pre-loaded with RNAlater, lysis solution, filter cartridges, collection tubes, nuclease-free water and elution solution
Isolation Technology Organic Extraction, Spin Column
Product Type Kit
Quantity 25 preps
Sample Size Up to 0.5mL whole blood
Sample Type Blood
Species Mouse
Target Total RNA, miRNA
Yield 4μg

For the purification of extremely high-quality total RNA (and optionally, microRNA) from mouse or rat blood in about 35 minutes. The Ambion kit includes sufficient reagents for 25 RNA purifications from whole blood samples ranging from 0.1–0.5 each.
Due to interference from the enzymatic inhibitors and anticoagulants present in blood samples, RNA purification from whole blood requires a stringent isolation method that yields high-quality RNA depleted of inhibiting agents and contaminants. The kit includes microfuge tubes preloaded with RNAlater™ Tissue Collection:RNA Stabilization Solution, an aqueous, nontoxic storage reagent that prevents RNA degradation and preserves RNA profiles within samples. Stabilized samples can be stored at room temperature for up to 3 days or at –20°C for long-term storage. The kit includes an alternative protocol for isolating total RNA that contains microRNA. Typically, 30–55 μg of total RNA (containing microRNA) is obtained from 0.5mL mouse blood. Yields from rat blood are considerably more variable, generally 18–40μg from 0.5mL blood.

  • Includes RNAlater™, predispensed in 2mL tubes, for RNA stabilization
  • Resulting RNA is suitable for stringent downstream applications such as array analysis and qRT-PCR

Not high-throughput compatible (Manual)

DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Blood, microRNA Isolation