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Invitrogen™ Ambion™ mMESSAGE mMACHINE™ T7 Transcription Kit DFS Item

in vitro synthesis of large amounts of capped RNA

Manufacturer: invitrogen™  AM1344M

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Catalog No. AM1344M


Description & Specifications


Concentration 40mM
For Use With (Application) Gene Expression Analysis and Genotyping, in vitro Transcription
Includes T7 Enzyme Mix, 10X Reaction Buffer, 2XNTP/CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, Gel Loading Buffer II, and Nuclease-free Water
Product Type Kit with manual
Quantity 25 reactions

mMESSAGE mMACHINE kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5' end.

  • RNA Yield: 20-30μg
  • Include cap analog [m7G(5')ppp(5')G] in ultra high-yield transcription reaction
  • Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution
  • Cap analog: GTP ratio of solution is 4:1, which is optimal for maximizing both RNA yield and the proportion of capped transcripts
  • For routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications
  • High yields are achieved by optimizing reaction conditions for RNA synthesis in presence of high nucleotide concentrations
  • RNA synthesized is protected from degradation by contaminating ribonucleases that present with RNase inhibitor - component of the Enzyme Mix