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Invitrogen™ Ambion™ mMESSAGE mMACHINE™ T7 Transcription Kit DFS Item

in vitro synthesis of large amounts of capped RNA

$580.00 - $590.00

Specifications

For Use With (Application) Gene Expression Analysis and Genotyping, in vitro Transcription
Includes T7 Enzyme Mix, 10X Reaction Buffer, 2XNTP/CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, Gel Loading Buffer II, and Nuclease-free Water
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Includes: T7 Enzyme Mix, 10X Reaction Buffer, 2XNTP/CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, Gel Loading Buffer II, Nuclease-free Water


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Specifications

Description

mMESSAGE mMACHINE kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5' end.

  • RNA Yield: 20-30μg
  • Include cap analog [m7G(5')ppp(5')G] in ultra high-yield transcription reaction
  • Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution
  • Cap analog: GTP ratio of solution is 4:1, which is optimal for maximizing both RNA yield and the proportion of capped transcripts
  • For routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications
  • High yields are achieved by optimizing reaction conditions for RNA synthesis in presence of high nucleotide concentrations
  • RNA synthesized is protected from degradation by contaminating ribonucleases that present with RNase inhibitor - component of the Enzyme Mix

Description & Specifications

Specifications

Gene Expression Analysis and Genotyping, in vitro Transcription
T7 Enzyme Mix, 10X Reaction Buffer, 2XNTP/CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, Gel Loading Buffer II, and Nuclease-free Water