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Invitrogen™ Ambion™ mMESSAGE mMACHINE™ SP6 Transcription Kit DFS Item


in vitro synthesis of large amounts of capped RNA

Manufacturer: invitrogen™  AM1340M

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Catalog No. AM1340M

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Description & Specifications

Specifications

For Use With (Application) Gene Expression Analysis and Genotyping, in vitro Transcription
Includes SP6 Enzyme Mix, 10X Reaction Buffer, 2XNTP/CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, Gel Loading Buffer II, and Nuclease-free Water
Product Type Kit with manual
Quantity 25 reactions

mMESSAGE mMACHINE™ kits are designed for in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5' end.

  • RNA Yield: 5-25μg
  • Include cap analog [m7G(5')ppp(5')G] in an ultra high-yield transcription reaction
  • Cap analog is incorporated only as first or 5' terminal G of transcript because its structure precludes its incorporation at other position in RNA molecule
  • Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution
  • Ideal for routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications