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Invitrogen™ Ambion™ MEGAshortscript™ T7 Transcription Kit DFS Item

Maximize the yield of short RNA transcripts

$386.40 - $396.90

Specifications

For Use With (Application) Gene Expression Analysis and Genotyping, in vitro Transcription
Includes T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-RNA 18S, TURBO DNase, Ammonium Acetate Stop Solution, Gel Loading Buffer II, and Nuclease-free Water
Storage Requirements T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-RNA 18S, TURBO DNase, Ammonium Acetate Stop Solution, and Gel Loading Buffer II are all stored at -20°C. Nuclease-free Water may be stored at any temperature.
View More Specs
Includes:  T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-RNA 18S, TURBO DNase, Ammonium Acetate Stop Solution, Gel Loading Buffer II, Nuclease-free Water

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Product Name Price Quantity    

AM1354

DFS Item
 
invitrogen™
AM1354
Each for $396.90

AM1354M

DFS Item
 
invitrogen™
AM1354M
Each for $386.40
Description & Specifications

Specifications

For Use With (Application) Gene Expression Analysis and Genotyping, in vitro Transcription
Includes T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-RNA 18S, TURBO DNase, Ammonium Acetate Stop Solution, Gel Loading Buffer II, and Nuclease-free Water
Storage Requirements T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-RNA 18S, TURBO DNase, Ammonium Acetate Stop Solution, and Gel Loading Buffer II are all stored at -20°C. Nuclease-free Water may be stored at any temperature.

The MEGAshortscript T7 Kit is designed for high yields of in vitro-transcribed RNA products, in the 20 to 500 nucleotide range. Using conventional methods, transcription of small templates often results in low mass yields of RNA. Short transcripts require many more transcription initiation events to synthesize a given mass of product. Since the initiation step is the rate-limiting step in transcription reactions, the yield of product from short templates is often low. The MEGAshortscript T7 Kit has been designed to overcome the drawbacks involved in conventional in vitro transcription to maximize the yield of short RNA transcripts.

  • Efficiently transcribes small templates (<300 bases) with consistently high yields
  • Designed for use with plasmid, synthetic oligonucleotide, or PCR product templates