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Invitrogen™ Ambion™ MAXIscript™ SP6 Transcription Kit DFS Item

Fast synthesis of RNA probes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations

Manufacturer: invitrogen™  AM1308

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Catalog No. AM1308


Description & Specifications


For Use With (Application) DNA and RNA Purification and Analysis, Gene Expression Analysis and Genotyping, in vitro Transcription, Northern Blotting, Nuclease Protection Assays, Nucleic Acid Gel Electrophoresis and Blotting, Southern Blotting
Includes SP6 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, pTRI-b-actin, and Nuclease-free Water
Product Type Kit
Quantity 30 reactions

Ambion MAXIscript SP6 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations. Each kit contains sufficient reagents for 30 reactions.

  • High yields of high specific activity, full-length RNA probes in just 10 minutes (RNA Yield: 2-6μg)
  • Ideal probe synthesis kit for nuclease protection assays, Northern and Southern blots, and in situ hybridization
  • Includes TURBO DNase
    Contain quality-tested DNase/RNase-free reagents
  • Synthesizes RNA probes with specific activities reaching 1 x 109 cpm/μg in just 10 minutes
  • Efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them perfect companion to ribonuclease protection assays
  • For synthesis of nonisotopically labeled probes
  • Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar™ Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into reaction
  • For synthesis of modest amounts of unlabeled RNA (2–6μg/20μL reaction) of up to 2kb