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Invitrogen™ Ambion™ MAXIscript™ SP6/T7 Transcription Kit DFS Item


Fast synthesis of RNA probes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations

Manufacturer: invitrogen™  AM1320

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Catalog No. AM1320

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Description & Specifications

Specifications

For Use With (Application) DNA and RNA Purification and Analysis, Gene Expression Analysis and Genotyping, in vitro Transcription, Northern Blotting, Nuclease Protection Assays, Nucleic Acid Gel Electrophoresis and Blotting, Southern Blotting
Includes SP6 Enzyme Mix, T7 Enzyme Mix,10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, pTRI-b-actin, and Nuclease-free Water
Product Type Kit
Quantity 30 reactions

Ambion™ MAXIscript™ SP6/T7 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations. Each kit contains sufficient reagents for 15 reactions of each enzyme.

  • High yields of high specific activity, full-length RNA probes in just 10 minutes
  • Ideal probe synthesis kit for nuclease protection assays, Northern and Southern blots, and in situ hybridization
  • Includes TURBO DNase™
  • Contain quality-tested DNase/RNase-free reagents
  • Synthesizes RNA probes with specific activities reaching 1 x 109 cpm/μg in just 10 minutes
  • Effcient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays
  • For synthesis of nonisotopically labeled probes
  • Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar™ Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into reaction
  • Used for synthesis of modest amounts of unlabeled RNA (2–6μg/20μL reaction) of up to 2kb