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Ambion™ M-MLV Reverse Transcriptase DFS Item


Commonly used in the first step of RT-PCR as well as for primer extension experiments

Manufacturer: life technologies  AM2044

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Catalog No. AM2044

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Description & Specifications

Specifications

For Use With (Application) RT-PCR, Primer extension experiments
Includes M-MLV Reverse Transcriptase (10,000 units); 500μL 10X First Strand Synthesis Buffer
Quantity 10,000 units
Sample Type ssRNA, ssDNA, RNA-DNA Hybrid
Storage Requirements Store at -20deg.C
Temperature (English) Operating 98.6deg.F
Temperature (Metric) Operating 37deg.C

Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is derived from a cloned region of the pol gene of M-MLV and isolated from an E. coli strain overexpressing this construct.

  • In the presence of a single-stranded RNA or DNA template, primer, and deoxyribonucleotides, M-MLV Reverse Transcriptase (RT) will extend the oligonucleotide primer, producing a complementary strand

  • Optimal reaction temperature: 37°C
  • Final product size: 7kb or less

PCR & Real-Time PCR, Reverse Transcription