Invitrogen™ In Vivo Factor VII Positive Control siRNA
Ambion™ In Vivo Factor VII Positive Control siRNA
Manufacturer: Invitrogen™ 4459408
DescriptionInvitrogen™ In Vivo siRNA Controls
What are Invitrogen™ In Vivo siRNAs?
Invitrogen™ In Vivo siRNAs are designed using the proven Silencer™ Select algorithm and incorporate chemical modifications for superior serum stability with in vivo delivery. The added serum stability does not compromise the performance of the siRNAs. Invitrogen™ In Vivo siRNAs have been shown to be non-toxic in vivo (mouse) and non-immunogenic (cell-based assays). In cell-based assays, Invitrogen™ In Vivo siRNAs exhibit equivalent or better potency than Silencer™ Select siRNAs. The result is better confidence in your in vivo RNAi experiments, better knockdown when paired with an efficient delivery solution, and the assurance of limited non-specific effects.
Factor VII (FVII) Positive Control siRNA
Invitrogen™ In Vivo Factor VII siRNA, In Vivo Ready, provides a positive control for experiments involving Invitrogen™ In Vivo siRNA delivery to mice. Factor VII (FVII⁄F7; also known as proconvertin) is a vitamin K-dependent serine protease that functions as a central protein in the coagulation cascade. FVII is synthesized in the liver and secreted to the plasma where it circulates in an inactive form. After delivery of FVII-targeting siRNA to the liver, knockdown can be measured by quantifying FVII protein levels in blood by robust, commercially available chromogenic assays. Moreover, blood samples can be collected from the same animal at multiple time points post-siRNA injection. These features enable much more rapid and efficient knockdown measurements for FVII than for gene targets that require quantitation of mRNA levels in liver tissue, making FVII an ideal control for in vivo siRNA delivery.
Invitrogen™ synthesizes and purifies each Invitrogen™ In Vivo siRNA in state-of-the-art facilities to meet the highest quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.
In Vivo Ready Controls
Our In Vivo Ready Control siRNAs are subjected to that extra level of purification and testing required for the introduction of siRNAs into animals. After HPLC purification and annealing, each siRNA is further purified utilizing a process that removes excess salt via a semi-permeable membrane. The result is highly pure siRNA with minimal salt content, suitable for in vivo applications. In vivo siRNAs are then sterile filtered, and tested for the presence of endotoxin. At concentrations of 50μM in presence of deionized water, in vivo Ready siRNAs contain <5.0 mM Na+, <0.06 mM K+, and <0.02 mM Mg2+.
|Ambion™ In-vivo Factor VII Positive Control siRNA, 50nmol, Unconjugated Dye, siRNA RNAi Type, Gene Knockdown Positive Control Type, Used for RNAi, Transfection, Factor VII Target Gene, –20°C Storage Temperature, For Research Use Only|
|The siRNA (annealed) is shipped at ambient temperature as dried down oligonucleotides in a single tube, along with 1.75mL nuclease-free water for resuspension|
|Ambion™ In-vivo Factor VII Positive Control siRNA|
For Research Use Only. Not for use in diagnostic procedures.