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Invitrogen™ Ambion™ DECAprime™ II DNA Labeling Kit DFS Item

Technically superior to existing methods

$325.50 - $821.10

Specifications

Product Type Kit, DNA Labeling
For Use With (Application) DNA and RNA Purification and Analysis, DNA Labeling, Northern Blotting, Nucleic Acid Gel Electrophoresis and Blotting, Nucleic Acid Labeling and Oligo Synthesis, Southern Blotting
Includes Exo– Klenow, 10X Decamer Solution, 5X Reaction Buffer minus dATP, 5X Reaction Buffer minus dCTP, DECAtemplate GAPDH-M, and 0.5 M EDTA, Nuclease-free water
Quantity 30 reactions
View More Specs
Includes:  Exo– Klenow, 10X Decamer Solution, 5X Reaction Buffer minus dATP, 5X Reaction Buffer minus dCTP, DECAtemplate™ GAPDH-M, 0.5 M EDTA, nuclease-free water.

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

AM1456

DFS Item
 
invitrogen™
AM1456
100 reactions Each for $821.10

AM1455

DFS Item
 
invitrogen™
AM1455
30 reactions Each for $325.50
Description & Specifications

Specifications

Product Type Kit, DNA Labeling
For Use With (Application) DNA and RNA Purification and Analysis, DNA Labeling, Northern Blotting, Nucleic Acid Gel Electrophoresis and Blotting, Nucleic Acid Labeling and Oligo Synthesis, Southern Blotting
Includes Exo– Klenow, 10X Decamer Solution, 5X Reaction Buffer minus dATP, 5X Reaction Buffer minus dCTP, DECAtemplate GAPDH-M, and 0.5 M EDTA, Nuclease-free water
Quantity 30 reactions

The Ambion kit is for labeling DNA using a random-priming method, which has technical improvements over existing methods including the use of a high-purity, exonuclease-free Klenow and Random Decamers to produce probes with greater than 109 cpm/μg in 10 min reactions. The kit includes sufficient reagents for 30 reactions.

Don't Trade Specific Activity for Yield

There is a trade-off between probe yield and probe specific activity when using the random-priming method for labeling DNA. Both limiting nucleotide and template mass affect probe yield. Until the labeled nucleotide becomes limiting, the larger the amount of DNA template used, the greater the yield of probe. However, once the labeled nucleotide becomes limiting, additional template will only result in lower specific activity, since the unlabeled template competes with the labeled probe for target.

Maximal Yields of High Specific Activity Probes
The DECAprime™ II DNA Labeling Kit produces probes with maximum specific activity even when the DNA template is impure or the quantity is unknown or very low. Data shows that low template amounts require long incubation times to reach maximum specific activity. Under these conditions, extended reaction times will increase both the yield and the specific activity of the probe.

Accessory Products:
The NucAway™ Spin Columns (Cat. No. AM10070) are recommended for probe purification.

  • Fast, 10 min reaction time
  • Elimination of Klenow exonuclease activity maximizes specific activity
  • Improved labeling kinetics maximizes yields
  • Flexible: both -dATP and -dCTP buffers supplied with each kit
  • Decamers produce greater primer-template stability

DNA and RNA Purification and Analysis, DNA Labeling, Northern Blotting, Nucleic Acid Gel Electrophoresis and Blotting, Nucleic Acid Labeling and Oligo Synthesis, Southern Blotting

Shipping Condition: Dry Ice