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Ambion™ CellsDirect™ Platinum™ SuperScript™ One Step qRT-PCR Kit DFS Item

No need for an RNA purification step

Manufacturer:  Life Technologies LS11753100

 View more versions of this product

Catalog No. 11753100


 

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Specifications Description & Specifications

Specifications

Primer-Probe Detection
10mL Resuspension Buffer; 1mL Lysis Enhancer; 500μL DNase I, Amplification Grade (1U/μL); 160μL 10X DNase I Buffer; 400μL 25mM EDTA; 100μL SuperScript III RT/Platinum Taq Mix; 2 x 1.25mL 2X Reaction Mix; 1mL 50mM MgSO4; 2mL DEPC-treated water; 10μL HeLa Total RNA (10ng/μL); 100μL ROX Reference Dye
Cells
7500 Fast System, 7500 System, BioRad MyiQ, BioRad iCycler iQ, BioRad iQ5, Cepheid SmartCycler, Corbett RotorGene, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
100 reactions
ROX Reference Dye (store at -20°C in the dark)