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Ambion™ CellsDirect™ Platinum™ SuperScript™ One Step qRT-PCR Kit DFS Item


No need for an RNA purification step

Manufacturer: life technologies  LS11753100

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 11753100

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Description & Specifications

Specifications

Includes 10mL Resuspension Buffer; 1mL Lysis Enhancer; 500μL DNase I, Amplification Grade (1U/μL); 160μL 10X DNase I Buffer; 400μL 25mM EDTA; 100μL SuperScript III RT/Platinum Taq Mix; 2 x 1.25mL 2X Reaction Mix; 1mL 50mM MgSO4; 2mL DEPC-treated water; 10μL HeLa Total RNA (10ng/μL); 100μL ROX Reference Dye
For Use With (Equipment) 7500 Fast System, 7500 System, BioRad MyiQ, BioRad iCycler iQ, BioRad iQ5, Cepheid SmartCycler, Corbett RotorGene, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Detection Method Primer-Probe Detection
Quantity 100 reactions
Sample Type Cells
Quantity 100 reactions
Storage Requirements ROX Reference Dye (store at -20°C in the dark)