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Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit DFS Item

No need for an RNA purification step

Manufacturer:  Invitrogen™ LS11753500

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Catalog No. 11753500



Description

Description

The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

  • Efficiency: offers significant time and cost savings for increased throughput
  • Convenience: enzyme mix includes both SuperScript™ III Reverse Transcriptase and Platinum™ Taq DNA Polymerase
  • Sensitivity: no separate RNA isolation step increases sensitivity by minimizing sample loss
  • Broad detection range: detect targets from samples ranging from a single cell to 10,000 cells
  • Versatility: compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)
Streamline your workflow In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.
Ideal for your qRT-PCR application
CellsDirect technology is validated for gene expression studies involving limited samples. CellsDirect One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps. The CellsDirect One-Step qRT-PCR Kit may also be used to detect genomic DNA targets. CellsDirect One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g., TaqMan™ or LUX™ fluorogenic primers).

This kit includes a separate tube of ROX™ Reference Dye. An equivalent kit with ROX Reference Dye included in the 2X Reaction Mix is also available.

Gene Expression Analysis and Genotyping, PCR and Real-Time PCR, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling, Real-Time PCR Directly from Samples

Specifications

Specifications

Primer-Probe Detection
Cells
• 10 ml Resuspension Buffer
• 1 ml Lysis Enhancer
• 2 × 1.25 ml DNase I, Amplification Grade (1 U/µl)
• 800 µl 10X DNase I Buffer
• 2 × 1 ml 25 mM EDTA
• 500 µl SuperScript® III RT/Platinum® Taq Mix
• 12.5 ml 2X Reaction Mix
• 1 ml 50 mM MgSO4
• 12.5 ml DEPC-treated water
• 10 µl HeLa Total RNA (10 ng/µl)
• 500 µl ROX™ Reference Dye (store at -20°C in the dark)
The kit is shipped on dry ice and should be stored at -20°C
Fast, Standard
7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4
500 reactions
1-Step qRT-PCR
CellsDirect™, Platinum®, SuperScript™
SDS
Documents

For Research Use Only. Not for use in diagnostic procedures.