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Ambion™ CellsDirect™ Platinum™ SuperScript™ One Step qRT-PCR Kit DFS Item


No need for an RNA purification step

Manufacturer: life technologies  11753500

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Catalog No. 11753500

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Description & Specifications

Specifications

Detection Method Primer-Probe Detection
For Use With (Equipment) 7500 Fast System, 7500 System, BioRad MyiQ, BioRad iCycler iQ, BioRad iQ5, Cepheid SmartCycler, Corbett RotorGene, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Includes 10mL Resuspension Buffer; 1mL Lysis Enhancer; 2 x 1.25mL DNase I, Amplification Grade (1U/μL); 800μL 10X DNase I Buffer; 2 x 1mL 25mM EDTA; 500μL SuperScript III RT/Platinum Taq Mix; 12.5mL 2X Reaction Mix; 1mL 50mM MgSO4; 12.5mL DEPC-treated water; 10μL HeLa Total RNA (10ng/μL); 500μL ROX Reference Dye
Quantity 500 reactions
Sample Type Cells
Storage Requirements ROX Reference Dye (store at -20°C in the dark)

The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

  • Efficiency: offers significant time and cost savings for increased throughput
  • Convenience: enzyme mix includes both SuperScript™ III Reverse Transcriptase and Platinum™ Taq DNA Polymerase
  • Sensitivity: no separate RNA isolation step increases sensitivity by minimizing sample loss
  • Broad detection range: detect targets from samples ranging from a single cell to 10,000 cells
  • Versatility: compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)
Streamline your workflow In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.
Ideal for your qRT-PCR application
CellsDirect technology is validated for gene expression studies involving limited samples. CellsDirect One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps. The CellsDirect One-Step qRT-PCR Kit may also be used to detect genomic DNA targets. CellsDirect One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g., TaqMan™ or LUX™ fluorogenic primers).

This kit includes a separate tube of ROX™ Reference Dye. An equivalent kit with ROX Reference Dye included in the 2X Reaction Mix is also available.

Gene Expression Analysis and Genotyping, PCR and Real-Time PCR, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling, Real-Time PCR Directly from Samples