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Ambion™ CellsDirect™ Platinum™ SuperScript™ One Step qRT-PCR Kit DFS Item


No need for an RNA purification step

Manufacturer: life technologies  LS11753500

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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Catalog No. 11753500

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Description & Specifications

Specifications

Includes 10mL Resuspension Buffer; 1mL Lysis Enhancer; 2 x 1.25mL DNase I, Amplification Grade (1U/μL); 800μL 10X DNase I Buffer; 2 x 1mL 25mM EDTA; 500μL SuperScript III RT/Platinum Taq Mix; 12.5mL 2X Reaction Mix; 1mL 50mM MgSO4; 12.5mL DEPC-treated water; 10μL HeLa Total RNA (10ng/μL); 500μL ROX Reference Dye
Sample Type Cells
Detection Method Primer-Probe Detection
Quantity 500 reactions
Storage Requirements ROX Reference Dye (store at -20°C in the dark)
For Use With (Equipment) 7500 Fast System, 7500 System, BioRad MyiQ, BioRad iCycler iQ, BioRad iQ5, Cepheid SmartCycler, Corbett RotorGene, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Quantity 500 reactions