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Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit DFS Item

No need for an RNA purification step

Manufacturer:  Invitrogen™ LS11753500

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Catalog No. 11753500



Description

Description

The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

  • Efficiency: offers significant time and cost savings for increased throughput
  • Convenience: enzyme mix includes both SuperScript™ III Reverse Transcriptase and Platinum™ Taq DNA Polymerase
  • Sensitivity: no separate RNA isolation step increases sensitivity by minimizing sample loss
  • Broad detection range: detect targets from samples ranging from a single cell to 10,000 cells
  • Versatility: compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)
Streamline your workflow In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.
Ideal for your qRT-PCR application
CellsDirect technology is validated for gene expression studies involving limited samples. CellsDirect One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps. The CellsDirect One-Step qRT-PCR Kit may also be used to detect genomic DNA targets. CellsDirect One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g., TaqMan™ or LUX™ fluorogenic primers).

This kit includes a separate tube of ROX™ Reference Dye. An equivalent kit with ROX Reference Dye included in the 2X Reaction Mix is also available.

Gene Expression Analysis and Genotyping, PCR and Real-Time PCR, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling, Real-Time PCR Directly from Samples

Specifications

Specifications

Primer-Probe Detection
500 Reaction
2X
1-Step qRT-PCR
CellsDirect™, Platinum™, SuperScript™
7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4
Cells
500 reactions
Fast, Standard
SDS
Documents

For Research Use Only. Not for use in diagnostic procedures.