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Ambion™ ArrayScript™ Reverse Transcriptase DFS Item


Suitable for RT and RT-PCR applications where high yields of full-length cDNA are required, such as cDNA library construction, 5' RLM-RACE, etc.

Manufacturer: life technologies  AM2049

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Catalog No. AM2049

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Description & Specifications

Specifications

For Use With (Application) RT and RT-PCR
Includes 10,000 units supplied with a 10X Reaction Buffer
Quantity 10,000 units
Sample Type ssRNA, ssDNA, RNA-DNA Hybrid
Storage Requirements Store at -20deg.C
Temperature (English) Operating 98.6deg.F
Temperature (Metric) Operating 37deg.C

  • Ambion ArrayScript Reverse Transcriptase is a M-MLV reverse transcriptase that has been engineered to produce high yields of full-length cDNA
  • 4000U are supplied along with a 10X Reaction Buffer. Reverse transcriptases (RTs) are used in a variety of molecular biology experiments where success can depend upon full-length cDNA synthesized during the reverse transcription reaction
  • In RNA amplification experiments, up to twice as much cRNA is synthesized when the first-strand cDNA is generated with ArrayScript RT compared to wild type M-MLV, AMV, or other engineered M-MLV reverse transcriptases when low amounts of total RNA (∽100ng) are used. This can eliminate the need to perform two rounds of cRNA amplification when working with limiting amounts of total RNA

Optimal reaction temperature: 37°C
Final product size: 7kb or less

Applications:

PCR & Real-Time PCR, Reverse Transcription