Ambion ANTI-RNase is a protein ribonuclease inhibitor that non-covalently binds and inactivates neutral pancreatic RNase A–type enzymes. No inhibition is seen with RNase 1, T1, T2, H, U1, U2, or CL3. ANTI-RNase is distinct from human placental ribonuclease inhibitor in that it has a more robust interaction with RNase and does not release active RNase in the absence of DTT or other reducing agents. ANTI-RNase may be used in any application where placental ribonuclease inhibitor is used. It is ideal for use in invitro transcription and translation, cDNA synthesis PCR, RT-PCR, RNA isolation procedures, and the preparation of RNase-free antibodies. ANTI-RNase is added to reactions to give a final concentration of 1U/μL. Slight inhibition of ANTI-RNase is measured in the presence of DTT (≤20mM). ANTI-RNase is thoroughly tested for contaminating RNase, exonuclease, and protease activity.
Unit Definition: One unit is the amount of protein required to inhibit the activity of 5ng RNase A by 50%.
Gene Expression Analysis and Genotyping, In Vitro Transcription, PCR and Real-Time PCR, Real Time PCR (qPCR), Reverse Transcription
Shipping Condition: Dry ice
|15 to 30U/μL|
|Non-covalently binds and inactivates neutral pancreatic RNase A–type enzymes|
|Store at -20°C. Do not store in a frost–free freezer or at -70°C.|