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Invitrogen™ Ambion™ ANTI-RNase (15-30U/μL) DFS Item


More robust RNase interaction over human placental ribonuclease inhibitor

Manufacturer: invitrogen™  AM2692

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Catalog No. AM2692

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Description & Specifications

Specifications

Concentration 15 to 30U/μL
For Use With (Application) Non-covalently binds and inactivates neutral pancreatic RNase A–type enzymes
Product Type ANTI-RNase
Quantity 10,000 units
Storage Requirements Store at -20°C. Do not store in a frost–free freezer or at -70°C.

Ambion ANTI-RNase is a protein ribonuclease inhibitor that non-covalently binds and inactivates neutral pancreatic RNase A–type enzymes. No inhibition is seen with RNase 1, T1, T2, H, U1, U2, or CL3. ANTI-RNase is distinct from human placental ribonuclease inhibitor in that it has a more robust interaction with RNase and does not release active RNase in the absence of DTT or other reducing agents. ANTI-RNase may be used in any application where placental ribonuclease inhibitor is used. It is ideal for use in invitro transcription and translation, cDNA synthesis PCR, RT-PCR, RNA isolation procedures, and the preparation of RNase-free antibodies. ANTI-RNase is added to reactions to give a final concentration of 1U/μL. Slight inhibition of ANTI-RNase is measured in the presence of DTT (≤20mM). ANTI-RNase is thoroughly tested for contaminating RNase, exonuclease, and protease activity.

Unit Definition: One unit is the amount of protein required to inhibit the activity of 5ng RNase A by 50%.

Gene Expression Analysis and Genotyping, In Vitro Transcription, PCR and Real-Time PCR, Real Time PCR (qPCR), Reverse Transcription