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Molecular Probes™ alamarBlue™ Cell Viability Reagent DFS Item

AlamarBlue™ Cell Viability Reagent

$416.00 - $826.00

Specifications

Cell Type Bacteria, Eukaryotic Cells, Fungal Cells
Color Blue
Content And Storage Store in refrigerator at 2°C to 8°C.
Description alamarBlue™ Cell Viability Reagent
Detection Method Colorimetric, Fluorescence
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
DAL1025 DFS Item
View Documents Promotion Details
Molecular Probes™
DAL1025
25 mL
Each for $416.00
 
DAL1100 DFS Item
View Documents Promotion Details
Molecular Probes™
DAL1100
100 mL
Each for $826.00
 
Description

Description

alamarBlue reagent is a redox indicator that yields a colorimetric change and a fluorescent signal in response to metabolic activity. It is a proven safe and non-toxic dye used for quantitative analysis of cell viability and cell proliferation, for cytokine bioassays and in vitro cytotoxicity studies.

Applications:
  • Cell proliferation determinations—alamarBlue reagent is water soluble, stable in culture, and non-toxic
  • Cell viability assays, including cytotoxicity—metabolic activity and dye generation changes in proportion to altered viability and cell damage
  • Cytokine assays—measure cytokine-induced proliferation, recover and expand cells at the end of the study if desired
alamarBlue reagent as a cell viability assay reagent
The alamarBlue assay is based on the ability of metabolically active cells to convert the reagent into a fluorescent and colorimetric indicator. Damaged and non-viable cells have lower innate metabolic activity, and generate a proportionally lower signal. The dye itself has low toxicity, and is generally safe for cells.

A safe, convenient, and versatile assay
alamarBlue reagent is an indigo-colored liquid that has been shown to effectively measure innate metabolic activity in animal, fungal and bacterial cells. Since alamarBlue reagent is non-toxic, the effector cells may be recovered for further analysis or cell expansion at the end of a study. alamarBlue reagent is water-soluble, saving time otherwise spend on washing and extraction steps. The homogeneous assay format of this kit is fast—add the single reagent directly to cells in suspension or to attached cells in full medium. The protocol is readily amenable to automation and high-throughput assays. The ability to detect the alamarBlue indicator by either fluorescence or spectroscopy provides compatibility with flow cytometry, plate readers and microscopy.

Experimental detection of alamarBlue reagent
  • Fluorometry: by excitation at 530–560 nm and emission at 590 nm
  • Spectrophotometry: by absorbance measurements at 570 nm and 600 nm
Specifications

Specifications

Bacteria, Eukaryotic Cells, Fungal Cells
Store in refrigerator at 2°C to 8°C.
Colorimetric, Fluorescence
Spectrophotometer, Microplate Reader
Resazurin
530-560 nm
96-well plate, Cuvettes, 384-well plate
alamarBlue™
High-throughput Compatible
Blue
alamarBlue™ Cell Viability Reagent
Viability Assay
Cell Viability Reagent
590 nm
Liquid
1 to 4 hr. (Up to 24 hr. for low cell numbers)
Wet Ice
SDS
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For Research Use Only. Not for use in diagnostic procedures.