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Molecular Probes™ alamarBlue™ Cell Viability Reagent DFS Item

AlamarBlue™ Cell Viability Reagent

Supplier:  Molecular Probes™ DAL1100

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Catalog No. DAL1100



Description

Description

The alamarBlue™ dye is a redox indicator that yields a colorimetric change and a fluorescent signal in response to metabolic activity. It is a proven safe and non-toxic dye used for quantitative analysis of cell viability and cell proliferation, for cytokine bioassays and in vitro cytotoxicity studies.

Applications:

  • Cell proliferation determinations—the alamarBlue™ dye is water soluble, stable in culture, and non-toxic
  • Cell viability assays, including cytotoxicity—metabolic activity and dye generation changes in proportion to altered viability and cell damage
  • Cytokine assays—measure cytokine-induced proliferation, recover and expand cells at the end of the study if desired

    The alamarBlue™ dye as a cell viability assay reagent
    The alamarBlue™ assay is based on the ability of metabolically active cells to convert the reagent into a fluorescent and colorimetric indicator. Damaged and non-viable cells have lower innate metabolic activity, and generate a proportionally lower signal. The dye itself has low toxicity, and is generally safe for cells.

    A safe, convenient, and versatile assay
    AlamarBlue™ dye is an indigo-colored liquid that has been shown to effectively measure innate metabolic activity in animal, fungal and bacterial cells. Since alamarBlue™ dye is non-toxic, the effector cells may be recovered for further analysis or cell expansion at the end of a study. AlamarBlue™ dye is water-soluble, saving time otherwise spend on washing and extraction steps. The homogeneous assay format of this kit is fast—add the single reagent directly to cells in suspension or to attached cells in full medium. The protocol is readily amenable to automation and high-throughput assays. The ability to detect the alamarBlue™ indicator by either fluorescence or spectroscopy provides compatibility with flow cytometry, plate readers and microscopy.

    Experimental detection of alamarBlue™ dye

  • Fluorometry: By excitation at 530–560 nm and emission at 590 nm
  • Spectrophotometry: By absorbance measurements at 570 nm and 600 nm
  • Specifications

    Specifications

    Bacteria, Eukaryotic Cells, Fungal Cells
    Store in refrigerator at 2°C to 8°C.
    Colorimetric, Fluorescence
    Spectrophotometer, Microplate Reader
    Resazurin
    530-560 nm
    96-well plate, Cuvettes, 384-well plate
    alamarBlue™
    Wet Ice
    Blue
    alamarBlue™ Cell Viability Reagent
    Viability Assay
    Cell Viability Reagent
    590 nm
    Liquid
    1 to 4 hr. (Up to 24 hr. for low cell numbers)
    100 mL
    High-throughput Compatible
    SDS
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    For Research Use Only. Not for use in diagnostic procedures.