missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

Molecular Probes™ alamarBlue™ Cell Viability Reagent DFS Item

AlamarBlue™ Cell Viability Reagent

Supplier:  Molecular Probes™ DAL1025

 View more versions of this product

Catalog No. DAL1025



alamarBlue reagent is a redox indicator that yields a colorimetric change and a fluorescent signal in response to metabolic activity. It is a proven safe and non-toxic dye used for quantitative analysis of cell viability and cell proliferation, for cytokine bioassays and in vitro cytotoxicity studies.


Cell proliferation determinations—alamarBlue reagent is water soluble, stable in culture, and non-toxic
Cell viability assays, including cytotoxicity—metabolic activity and dye generation changes in proportion to altered viability and cell damage
Cytokine assays—measure cytokine-induced proliferation, recover and expand cells at the end of the study if desired

alamarBlue reagent as a cell viability assay reagent
The alamarBlue assay is based on the ability of metabolically active cells to convert the reagent into a fluorescent and colorimetric indicator. Damaged and non-viable cells have lower innate metabolic activity, and generate a proportionally lower signal. The dye itself has low toxicity, and is generally safe for cells.

A safe, convenient, and versatile assay
alamarBlue reagent is an indigo-colored liquid that has been shown to effectively measure innate metabolic activity in animal, fungal and bacterial cells. Since alamarBlue reagent is non-toxic, the effector cells may be recovered for further analysis or cell expansion at the end of a study. alamarBlue reagent is water-soluble, saving time otherwise spend on washing and extraction steps. The homogeneous assay format of this kit is fast—add the single reagent directly to cells in suspension or to attached cells in full medium. The protocol is readily amenable to automation and high-throughput assays. The ability to detect the alamarBlue indicator by either fluorescence or spectroscopy provides compatibility with flow cytometry, plate readers and microscopy.

Experimental detection of alamarBlue reagent

Fluorometry: by excitation at 530–560 nm and emission at 590 nm
Spectrophotometry: by absorbance measurements at 570 nm and 600 nm


Bacteria, Eukaryotic Cells, Fungal Cells
Store in refrigerator at 2°C to 8°C.
Colorimetric, Fluorescence
Spectrophotometer, Microplate Reader
530-560 nm
96-well plate, Cuvettes, 384-well plate
Wet Ice
alamarBlue™ Cell Viability Reagent
Viability Assay
Cell Viability Reagent
590 nm
1 to 4 hr. (Up to 24 hr. for low cell numbers)
25 mL
High-throughput Compatible




For Research Use Only. Not for use in diagnostic procedures.