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AKT1 (PKB alpha) Recombinant Human Protein DFS Item

AKT1 (PKB alpha) Recombinant Human Protein

Manufacturer:  Life Technologies LSPV3599

 View more versions of this product

Catalog No. PV3599

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Description

Description

AKT1 (PKB alpha) is a serine⁄threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells.
Specifications

Specifications

NP_005154
Protein assays and analysis
Frozen
AKT1 (PKB alpha) Recombinant Human Protein
1mg
For research use only. Not for use in diagnostic procedures
AKT1 (PKB alpha)
AKT1 (PKB alpha) was pre-diluted in enzyme dilution buffer and assayed in 25mM Tris (pH 7.5), 10mM MgCl2, 0.5mM Na3VO4, 1mM DTT, 0.01% Triton™ X-100, 200μM ATP, 50μM crosstide peptide substrate (GRPRTSSFAEG) and trace [32P]-γ-ATP for 10 minutes at 30°C
Baculovirus
AKT1 (PKB alpha) Recombinant Human Protein
Human
0.26mg/mL
Frozen
59.1kDa
90% by SDS-PAGE
–80°C
1 Each
AKT1 (PKB alpha) Recombinant Human Protein
Kinase assay
AKT
630nmoles of phosphate transferred to Crosstide peptide substrate (GRPRTSSFAEG) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 2μg/mL
His Tag
Documents

For Research Use Only. Not for use in diagnostic procedures.