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AKT1 (PKB alpha) Recombinant Human Protein DFS Item

AKT1 (PKB alpha) Recombinant Human Protein

Manufacturer:  Life Technologies LSP2999

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Catalog No. P2999



Description

Description

AKT1 (PKB alpha) is a serine⁄threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells.
Specifications

Specifications

NP_005154
Cell viability, proliferation and function, pharma and biopharma, protein assays and analysis, target and lead identification and validation
59.1kDa
90% by SDS-PAGE
–80°C
1 Each
AKT1 (PKB alpha)
AKT1 (PKB alpha) was pre-diluted in enzyme dilution buffer and assayed in 25mM Tris (pH 7.5), 10mM MgCl2, 0.5mM Na3VO4, 1mM DTT, 0.01% Triton™ X-100, 200μM ATP, 50μM Crosstide peptide substrate (GRPRTSSFAEG) and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinases and inhibitors
AKT kinase family
220nmoles of phosphate transferred to Crosstide peptide substrate (GRPRTSSFAEG) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 2μg/mL
His Tag
0.24mg/mL
207
AKT1 (PKB alpha) Recombinant Human Protein
10μg
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
AKT1 (PKB alpha) Recombinant Human Protein
Kinase assay
Baculovirus
AKT1 (PKB alpha) Recombinant Human Protein
Human
Documents

For Research Use Only. Not for use in diagnostic procedures.