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Shrimp Alkaline Phosphatase (SAP)


Treat unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. Shrimp Alkaline Phosphatase (SAP) is useful in molecular biology applications such as the dephosphorylation of phosphorylated ends of DNA or RNA for subsequent use in cloning or end-labeling of probes.

Manufacturer: life technologies  LS783905000UN

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Catalog No. AF783905000

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Description & Specifications

Specifications

For Use With (Application) Dephosphorylation of DNA prior to cloning
Quantity 5000 units

  • 100% heat-inactivated in 5 min at 65°C
  • Significantly improved storage stability at lower temperatures
  • Very high specific activity (see Fig. 3)
  • Removes 5’-phosphates from DNA, RNA, dNTPs, and proteins
  • Purified from a recombinant source
  • May be added directly to restriction enzyme digests
  • No vector purification necessary
  • Requires no supplemental zinc or other additives for activity
  • Works direct in many different buffers
  • Easy treatment of unincorporated dNTPs in PCR products prior to DNA sequencing or SNP analysis