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Affymetrix™ Shrimp Alkaline Phosphatase (SAP)

Treat unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. Affymetrix™ Shrimp Alkaline Phosphatase (SAP) is useful in molecular biology applications such as the dephosphorylation of phosphorylated ends of DNA or RNA for subsequent use in cloning or end-labeling of probes.

Manufacturer: affymetrix™  78390500UN

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Catalog No. AF78390500


Description & Specifications


Quantity 500 units
For Use With (Application) Dephosphorylation of DNA prior to cloning

  • 100% heat-inactivated in 5 min at 65°C
  • Significantly improved storage stability at lower temperatures
  • Very high specific activity (see Fig. 3)
  • Removes 5’-phosphates from DNA, RNA, dNTPs, and proteins
  • Purified from a recombinant source
  • May be added directly to restriction enzyme digests
  • No vector purification necessary
  • Requires no supplemental zinc or other additives for activity
  • Works direct in many different buffers
  • Easy treatment of unincorporated dNTPs in PCR products prior to DNA sequencing or SNP analysis