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Thermo Scientific™ Adenovirus Fiber (Monomer and Trimer) Ab-4, Mouse Monoclonal Antibody

Obtain accurate, reproducible results in western blotting and immunofluorescence experiments.

Manufacturer: thermo scientific™  MS1027P1ABX

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Catalog No. MS1027P1ABX

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Description & Specifications


Antigen Adenovirus Fiber (Monomer and Trimer) Ab-4
Applications Immunofluorescence
Applications Western Blotting
Classification Monoclonal
Clone 4D2
Concentration 1mg/mL
Conjugate Unlabeled
Cross Reactivity Adenovirus
Format Concentrated
Host Species Murine
Immunogen UV-irradiated Ad2 virus
Isotype IgG2a kappa
Quantity 100μL
Regulatory Status RUO
Research Discipline Infectious Disease
Type Primary
Monoclonal or Polyclonal Monoclonal
Target Species Adenovirus

Obtain accurate, reproducible results in western blotting and immunofluorescence experiments with Thermo Scientific™ Adenovirus Fiber (Monomer and Trimer) Ab-4, Mouse Monoclonal Antibody.

Adenovirus capsid proteins are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base, and fiber) are synthesized late in infection. Fiber plays a crucial role in adenovirus infection by attaching the virus to a specific receptor on the cell surface. Ad2 and Ad5 fibers are proposed to consist of three domains: an N-terminal tail that interacts with penton base, a shaft composed of 22 repeats of a 15 amino acid segment that forms β-sheet and β-bends, and a knob at the C-terminus that contains the type-specific antigen and is responsible for binding to the cell surface receptor. It is shown that the fiber of Ad2 is most likely a trimer when found on the viron.

Host Species: Mouse

Clone: 4D2

Isotype: IgG2a/κ

Species Reactivity: Adenovirus.

Epitope: N-terminus, probably the first 17aa

Immunogen: UV-irradiated Ad2 virus

Molecular Weight: 62kDa (monomer) and 180-200kDa (trimer)

Positive Control: Adenovirus infected cells and tissues

Cellular Localization: Nuclear Ab-4 recognizes both monomers and trimers, as judged by reactivity with both boiled (monomers [62kDa] only) and unboiled (both monomers and trimers [180-200kDa bands]) fiber on Western blots and by indirect immunofluorescence studies of cells infected with a temperature-sensitive Ad5 fiber mutant (H5ts142) which does not form trimers at the nonpermissive temperature at both 32°C and 39.5°C.

Recommended for:

  • Western Blotting
  • Immunofluorescence
  • Inhibition of Ad2 attachment (Not suitable)


USA: RUO; Int'l: RUO