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Thermo Scientific™ Acclaim™ PepMap™ 100 C18 LC Columns

Feature high loading capacity for exceptional suitability in the analysis of low abundant peptides in complex proteomics samples.

$405.22 - $1,857.74

Specifications

Endcapped Yes
Packing Material Spherical, fully porous ultrapure silica
pH 2 to 8
Pore Size 100Å
Stationary Phase C18
View More Specs

 Disclaimers

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

Products
Catalog Number Mfr. No. Column Format Diameter (Metric) Length (Metric) Particle Size Price Quantity    

DX164261

 
thermo scientific™
164261
Analytical Column 0.075mm 250mm 3µm Each for $1,300.60

DX164567

 
thermo scientific™
164567
Analytical Column, NanoViper 0.075mm 50mm 3µm Each for $1,451.12

DX164568

 
thermo scientific™
164568
Analytical Column, NanoViper 0.075mm 150mm 3µm Each for $1,451.12

DX164569

 
thermo scientific™
164569
Analytical Column, NanoViper 0.075mm 250mm 3µm Each for $1,581.80

DX164570

 
thermo scientific™
164570
Analytical Column, NanoViper 0.075mm 500mm 3µm Each for $1,798.29

DX164572

 
thermo scientific™
164572
Analytical Column, NanoViper 1mm 150mm 3µm Each for $1,082.48

DX160454

 
thermo scientific™
160454
Trap Cartridge 0.3mm 5mm 5µm Each for $693.94

DX164197

 
thermo scientific™
164197
Trap Column 0.1mm 10mm 5µm Pack of 2 Each for $471.40

DX164213

 
thermo scientific™
164213
Trap Column 0.2mm 20mm 5µm Pack of 2 Each for $405.22

DX164199

 
thermo scientific™
164199
Trap Column 0.1mm 20mm 5µm Pack of 2 Each for $505.51

DX160323

 
thermo scientific™
160323
Analytical Column 0.075mm 150mm 5µm Each for $1,132.28

DX160326

 
thermo scientific™
160326
Analytical Column 0.075mm 250mm 5µm Each for $1,298.23

DX164562

 
thermo scientific™
164562
Analytical Column, NanoViper 0.05mm 150mm 2µm Each for $1,430.03

DX164537

 
thermo scientific™
164537
Analytical Column, NanoViper 0.3mm 150mm 2µm Each for $1,116.28

DX164534

 
thermo scientific™
164534
Analytical Column, NanoViper 0.75mm 150mm 2µm Each for $1,430.03

DX164561

 
thermo scientific™
164561
Analytical Column, NanoViper 0.05mm 50mm 2µm Each for $1,430.03

DX164560

 
thermo scientific™
164560
Analytical Column, NanoViper 0.3mm 50mm 2µm Each for $1,116.28

DX164536

 
thermo scientific™
164536
Analytical Column, NanoViper 0.75mm 250mm 2µm Each for $1,631.18

DX164535

 
thermo scientific™
164535
Trap Column, NanoViper 0.075mm 20mm 3µm Pack of 2 Each for $767.78

DX164571

 
thermo scientific™
164571
Analytical Column, NanoViper 0.3mm 150mm 3µm Each for $1,082.48

DX164563

 
thermo scientific™
164563
Analytical Column, NanoViper 0.75mm 50mm 2µm Each for $1,430.03

16-494-2

 
thermo fisher scientific
164942
nanoViper FS 0.075mm 500mm 2µm Each for $1,857.74

16-494-6

 
thermo scientific™
164946
Trap Cartridge 75µm 20mm 3µm Pack for $783.81

DX160321

 
thermo scientific™
160321
Analytical Column 0.075mm 150mm 3µm Each for $1,202.82
Description & Specifications

Specifications

Endcapped Yes
Packing Material Spherical, fully porous ultrapure silica
pH 2 to 8
Pore Size 100Å
Stationary Phase C18
Surface Area 450m²/g
Temperature 60°C
USP Type L1

Generate high-resolution analyses of tryptic, natural, and synthetic peptides using Thermo Scientific™ Acclaim™ PepMap™ 100 C18 LC Columns. These columns are often used for LC-MS/MS peptide mapping for protein identification, biomarker discovery, and systems biology. Due to their high loading capacity, Acclaim PepMap 100 C18 columns are exceptionally suitable for the analysis of low abundant peptides in complex proteomics samples. Most of these C18 columns are pre-assembled with Thermo Scientific™ nanoViper™ Fingertight Fittings for easy installation. Classic columns are available as well.

Column Fittings

  • Analytical columns are supplied with a standard 1/32" fitting at inlet
  • nanoViper columns are supplied with a nanoViper fitting at inlet and has 10 mm usable fused silica on the outlet
  • nanoViper FS columns are supplied with a nanoViper fitting at inlet and has 45 mm usable fused silica at outlet for greater compatibility with Peek sleaves and microtight unions
Key advantages of nanoViper fittings
  • Ease of use, no sleeve for small capillaries
  • Tool-free assembly
  • No column damage from overtightening: capable of withstanding pressures up to 1000 bars and is compatible with third party valves and unions
  • No experimental failure due to bad connections
Ideal for high-resolution peptide mapping of tryptic, natural and synthetic peptides as well as LC-MS/MS peptide mapping for protein identification, biomarker discovery and systems biology.
  • High loading capcity
  • Use for the analysis of low abundant peptides often found in complex proteomics samples
  • Select the column length and I.D. for your throughput, sensitivity and amount of sample available requirements
  • Choose from 50, 150, 250, 500mm lengths and 50, 75, 300 and 1000µm I.D.
  • Superior protein and peptide separations
  • Trifluoroacetic acid (TFA)-free LC/MS minimizes suppression effects for enhanced MS sensitivity
  • Outstanding separation efficiency
  • Superior resolution and recovery
  • Easy installation with nanoViper™ Fingertight Fittings

PepMap nano columns are also available pre-assembled with Thermo Scientific™ Dionex™ nanoViper™ Fingertight Fitting for a dead-volume-free connection to 1000 bar.

  • Requires no tools
  • Provides virtually zero-dead-volume connections
  • Withstands pressures up to a 1000 bar
About Nano, capillary, and micro Acclaim PepMap columns

Nano (75µm), capillary (300µm) and micro (1000µm) columns are ideal for protein and peptide analyses. Sample amount is often limited and smaller I.D. columns can increase in sensitivity in sample-limited situations. While nano LC columns are often used in proteomics, the concentrating effects of capillary and micro columns are beneficial for metabolomics or for facilitating LC-MS interfacing. Thermo Scientific Acclaim PepMap columns are offered in nano, capillary, and micro column in a variety of chemistries for peptides and protein analysis.

Use PepMap Trap columns for desalting and preconcentration of peptides before LC separation with MS detection. The trap columns provide the highest efficiencies for one dimensional peptide mapping experiments and 2D-LC analyses.