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ABsolute qPCR SYBR Green Mixes

Simplify reaction setup with these optimized 2x qPCR master mixes containing all reagents for qPCR with the exception of template and primers.

Manufacturer: abgene inc usa  AB1159B

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Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Catalog No. AB-1159B

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Description & Specifications


Includes ABsolute qPCR Buffer (Thermo-Start DNA polymerase, dNTPs, MgCl2, SYBR Green I, ROX™ passive reference dye (only in ROX mixes), Fluorescein (only in Fluorescein mix)); Separate MgCl2; Separate ROX vial (only in kits indicated)
Storage Requirements -20°C Avoid freeze/thaw cycles
Activation Time heated to 95°C for 15 minutes
Description ABsolute QPCR SYBR Green Mix
Quantity 2000 x 50µL rxns

ABsolute qPCR SYBR Green Mixes are optimized for SYBR Green chemistry and contain all the components necessary to perform quantitative PCR, with the exception of template and primers. The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or a separate ROX vial. A version with no reference dye is also available as well as a master mix optimized for capillary instruments.


  • High Sensitivity & Specificity -The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
  • Optimised buffer containing SYBR Green I
  • Reproducibility -All master mixes undergo extensive QC to ensure batch to batch consistency
  • Broad dynamic range