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ABsolute™ qPCR Master Mixes, SYBR Green-Based Detection

Thermo Scientific™ ABsolute qPCR Master Mixes offer sensitivity and reproducibility for real-time PCR applications.

Manufacturer:  Thermo Fisher Scientific AB1285B

Catalog No. AB1285B

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.


Description

Description

Enjoy sensitivity and reproducibility in real-time PCR applications with Thermo Scientific™ ABsolute™ SYBR Green-Based Detection qPCR Master Mixes.

ABsolute qPCR Master Mixes are designed to achieve the most consistent and reproducible data across all qPCR platforms. Proprietary additives give enhanced and consistent end point readings as well as low Ct values. All mixes contain Thermo-Start Taq DNA Polymerase, for increased specificity, and contain dTTP for increased reaction efficiency and sensitivity (low Ct).
  • Maximum specificity—Chemically-modified Thermo-Start™ Taq remains completely inactive until heated to 95°C for 15 minutes, thereby preventing nonspecific amplification

  • Improved efficiency—The use of dTTP in place of dUTP increases reaction efficiency by approximately 5% and therefore reduces reaction time

  • Increased sensitivity—The combination of Thermo-Start Taq and dTTP increases sensitivity and allows for detection of low copy number templates

  • Instrument specific—Individually optimized qPCR mixes designed for all major qPCR / Real-Time PCR instruments

  • Reproducible results—All mixes undergo extensive quality control testing to ensure batch-to-batch consistency

  • Simple protocol—Master mix format reduces protocol steps and minimizes risk of pipetting errors and sample contamination

Includes:

ABsolute qPCR Buffer (Thermo-Start DNA polymerase, dNTPs, MgCl2, SYBR Green I, ROX™ passive reference dye (only in ROX mixes), Fluorescein (only in Fluorescein mix)); Separate MgCl2; Separate ROX vial (only in kits indicated)

Recommended for:

Ideal for QPCR analysis in molecular biology applications, including gene expression and SNP typing

Specifications

Specifications

ABsolute QPCR SYBR Green Capillary Mix
ABsolute qPCR Buffer (Thermo-Start DNA polymerase, dNTPs, MgCl2, SYBR Green I, ROX™ passive reference dye (only in ROX mixes), Fluorescein (only in Fluorescein mix)); Separate MgCl2; Separate ROX vial (only in kits indicated)
-20°C Avoid freeze/thaw cycles
heated to 95°C for 15 minutes
2000 x 20µL rxn
SDS
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Product Certifications
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Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.