ABsolute™ qPCR Mix, SYBR Green, ROX
Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.
Manufacturer: Abgene Inc Usa AB1285/B
The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.
ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or no reference dye. A master mix optimized for capillary instruments is also available.
Maximum specificity—Chemically-modified Thermo-Start™ Taq remains completely inactive until heated to 95°C for 15 minutes, thereby preventing nonspecific amplification
Improved efficiency—The use of dTTP in place of dUTP increases reaction efficiency by approximately 5% and therefore reduces reaction time
Increased sensitivity—The combination of Thermo-Start Taq and dTTP increases sensitivity and allows for detection of low copy number templates
Instrument specific—Individually optimized qPCR mixes designed for all major qPCR / Real-Time PCR instruments
Reproducible results—All mixes undergo extensive quality control testing to ensure batch-to-batch consistency
Simple protocol—Master mix format reduces protocol steps and minimizes risk of pipetting errors and sample contamination
ABsolute qPCR Buffer (Thermo-Start DNA polymerase, dNTPs, MgCl2, SYBR Green I, ROX™ passive reference dye (only in ROX mixes), Fluorescein (only in Fluorescein mix)); Separate MgCl2; Separate ROX vial (only in kits indicated)
Ideal for QPCR analysis in molecular biology applications, including gene expression and SNP typing
|heated to 95°C for 15 minutes|
|ABsolute qPCR Buffer (Thermo-Start DNA polymerase, dNTPs, MgCl2, SYBR Green I, ROX™ passive reference dye (only in ROX mixes), Fluorescein (only in Fluorescein mix)); Separate MgCl2; Separate ROX vial (only in kits indicated)|
|-20°C Avoid freeze/thaw cycles|
|ABsolute QPCR SYBR Green Capillary Mix|
|2000 x 20µL rxn|