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Thermo Scientific™ ABsolute qPCR SYBR Green Mixes


Simplify reaction setup with these optimized 2x qPCR master mixes containing all reagents for qPCR with the exception of template and primers.

Manufacturer: thermo scientific™  AB-1220/A

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 Disclaimers

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Catalog No. AB1220A

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Description & Specifications

Specifications

Activation Time heated to 95°C for 15 minutes
Description ABsolute QPCR SYBR Green Fluorescein Mix
Includes ABsolute qPCR Buffer (Thermo-Start DNA polymerase, dNTPs, MgCl2, SYBR Green I, ROX™ passive reference dye (only in ROX mixes), Fluorescein (only in Fluorescein mix)); Separate MgCl2; Separate ROX vial (only in kits indicated)
Quantity 200 x 50µL rxns
Storage Requirements -20°C Avoid freeze/thaw cycles

ABsolute qPCR SYBR Green Mixes are optimized for SYBR Green chemistry and contain all the components necessary to perform quantitative PCR, with the exception of template and primers. The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or a separate ROX vial. A version with no reference dye is also available as well as a master mix optimized for capillary instruments.

ABsolute qPCR Master Mixes are designed to achieve the most consistent and reproducible data across all qPCR platforms. Proprietary additives give enhanced and consistent end point readings as well as low Ct values. All mixes contain Thermo-Start Taq DNA Polymerase, for increased specificity, and contain dTTP for increased reaction efficiency and sensitivity (low Ct).

  • Maximum specificity—Chemically-modified Thermo-Start™ Taq remains completely inactive until heated to 95°C for 15 minutes, thereby preventing nonspecific amplification

  • Improved efficiency—The use of dTTP in place of dUTP increases reaction efficiency by approximately 5% and therefore reduces reaction time

  • Increased sensitivity—The combination of Thermo-Start Taq and dTTP increases sensitivity and allows for detection of low copy number templates

  • Instrument specific—Individually optimized qPCR mixes designed for all major qPCR / Real-Time PCR instruments

  • Reproducible results—All mixes undergo extensive quality control testing to ensure batch-to-batch consistency

  • Simple protocol—Master mix format reduces protocol steps and minimizes risk of pipetting errors and sample contamination

Includes:

ABsolute qPCR Buffer (Thermo-Start DNA polymerase, dNTPs, MgCl2, SYBR Green I, ROX™ passive reference dye (only in ROX mixes), Fluorescein (only in Fluorescein mix)); Separate MgCl2; Separate ROX vial (only in kits indicated)

Recommended for:

Ideal for QPCR analysis in molecular biology applications, including gene expression and SNP typing